Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

我们立志成为全球首屈一指的研究密集型生物制药公司

130 年来，默沙东一直在为生命发明，为世界上许多最具挑战性的疾病提供药物和疫苗，以追求我们拯救和改善生命的使命。我们通过影响深远的政策、计划和合作伙伴关系来增加获得医疗保健的机会，以此证明我们对患者和人口健康的承诺。今天，我们继续站在研究的最前沿，以预防和治疗威胁人类和动物的疾病——包括癌症、传染病（如艾滋病毒和埃博拉病毒）以及新出现的动物疾病。

我们相信企业责任是关于我们对世界各地人民和社区的健康、社会、环境和经济影响。支持世界各地的社会、人民和社区是我们长期成功的基础。

我们奔向而不是来自世界上最大的健康挑战，从为世界上一些最令人衰弱的疾病寻找解决方案，到为有需要的人提供药物和疫苗，再到建立更有效的卫生系统，我们始终肩负着创造更美好世界的使命。

我们的努力分为四个主要领域：

获得健康

数以百万计的人活得更久、更富有成效，部分原因在于更好的医疗保健和创新药物和疫苗的获取。然而，需要取得更多进展，以确保在世界范围内更多地获得健康。

雇员

我们的员工是我们最重要的资产，也是我们发明背后的核心。

环境

一个健康的地球对人类健康和我们业务的可持续性至关重要。

道德与价值观

我们的声誉以及利益相关者对我们的信任取决于我们作为一家企业的行为方式。我们坚定不移地致力于维护最高的道德和价值观标准。